Infection of the wound bed is the 2nd most common cause of  Graft loss following split skin graft, next to hematoma(1). Wound bed infections increase the duration of hospital stay, increased cost of treatment, prolonged treatment and treatment failure. Hence identifying wound bed infection and treating it becomes the primary duty of the surgeon before applying skin graft for any wounds.  In the current setting, wound biopsy and culture is the important Modality through which wound infection is confirmed. Though Wound biopsy is gold standard in identifying wound infection, it is time consuming which further delays the management  of the wound. In this article we would like to report about the use of a real time fluorescent imaging in rapid identification of bacterial infections in wound bed.