Polarised infrared light enables enhancement of histo-morphological diagnosis of prostate cancer
Giorgi Kochiashvili, Alexandre Khuskivadze, Besarion Partsvania, Ketevan Chubinidze
Abstract
Background
In general, histomorphologic examination of a prostate tissue is necessary after the prostatectomy.
Aims
This study was carried out to investigate a possibility of the usage of polarized light for the formation and analyse of infrared images of prostate cancer.
Methods
Experiments were carried out in isolated prostates. For the obtaining of prostate infrared images a light source in the spectral range of 840–900nm was used. Infrared light polarization measurement was performed using polarizers working in 700–2000nm. Infrared polarized light incident on a CCD camera matrix was converted into electrical signals and sent to the PC for the creating visible image.
Specially elaborated software converts the electrical signals, received from the CCD camera, from near infrared (NIR) into visible image, that allows us to discriminate infrared images of healthy tissue from the malignant ones.
Results
It is shown that the intensity of near infrared (NIR) light passing through the cancerous outgrowth is lower than the intensity of NIR light passing through the non-cancerous tissue and the cancerous formations are differentiated as the dark areas in the relatively white background.
Conclusion
It has been shown that the utilization of polarized NIR light for prostate cancer targeting and visualization is a promising imaging modality for the discrimination of malignant areas in prostatectomy specimens.
Full Text:
In general, histomorphologic examination of a prostate tissue is necessary after the prostatectomy.
Aims
This study was carried out to investigate a possibility of the usage of polarized light for the formation and analyse of infrared images of prostate cancer.
Methods
Experiments were carried out in isolated prostates. For the obtaining of prostate infrared images a light source in the spectral range of 840–900nm was used. Infrared light polarization measurement was performed using polarizers working in 700–2000nm. Infrared polarized light incident on a CCD camera matrix was converted into electrical signals and sent to the PC for the creating visible image.
Specially elaborated software converts the electrical signals, received from the CCD camera, from near infrared (NIR) into visible image, that allows us to discriminate infrared images of healthy tissue from the malignant ones.
Results
It is shown that the intensity of near infrared (NIR) light passing through the cancerous outgrowth is lower than the intensity of NIR light passing through the non-cancerous tissue and the cancerous formations are differentiated as the dark areas in the relatively white background.
Conclusion
It has been shown that the utilization of polarized NIR light for prostate cancer targeting and visualization is a promising imaging modality for the discrimination of malignant areas in prostatectomy specimens.
PDF