Acute genital ulcers (AGU), also known as acute vulvar ulcers or Lipschütz ulcers, is an uncommon, non-sexually transmitted condition characterized by the sudden onset of painful necrotic ulcerations of the vulva or lower vagina. Their aetiology and pathogenesis are still unknown. They may be preceded by influenza-like symptoms, such as fever, headache or respiratory symptoms. We presented a case of pubertal girl with multiple painful ulcers on the vulva who visited our hospital. Other causes of vulvar ulcers were excluded by history taking, laboratory tests, and skin biopsy. We presented a case report of pubertal girl with non-sexually related AGU.