Rates of clarithromycin resistance in Helicobacter pylori sampled from healthy subjects in Cheonan, Korea

Young Sam Yuk, Ga-Yeon Kim


The increasing use of the standard triple therapy for eradication of Helicobacter pylori (H. pylori) has led to an increase in the prevalence of strains resistant to existing drugs, thereby lowering the success rate of eradication therapies.

This study aimed at promoting effective eradication therapy by investigating the H. pylori infection rate, incidence of clarithromycin resistance, and types of mutations.

Using a PCR kit that amplifies a gene site known to be resistant to antibiotics in H. pylori, the resistance gene retention rate is determined and analyzed using various methods. The rapid urease test (RUT) was performed on patients undergoing routine health exams in Cheonan, and residual specimens were analyzed through DPO-based multiplex PCR to examine point mutations of the 23S rRNA gene, a gene responsible for clarithromycin resistance in H. pylori.

The statistical program R was used for data analysis. Data are presented as medians and ranges. A chi-square test was used to analyze the categorical data. A p-value
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